According ETEC were the dominant types from Khairpur,

According to Mufti, P., et al.,  dearth of information on STEC infections in
Pakistan, and to date, of 99 diarrhea patients there are only two reports
found  positive for  STEC isolates (Mufti, P., et al.,
1999).From
Faisalabad Multiplex PCR results showed that 22 (11%) of the patients were
positive for stx
genes.Among other virulence factors, plasmid borne hly and eae genes
were found in 13 (6.5 %) and 16 (8.0 %) patients respectively.However, only 14
STEC isolates were identified.The stx1, stx2, hly
and eae genes
were positive 11(78.5%), 11(78.5%), 3(21.4%) and 6 (42.8%) respectively.Only
three (3) of these isolates belonged to serotype O157 (Mashkoor, Mohsin.,
2007).In another study conducted in Faisalabad in which 23 putative E. coli
isolates, five (21.7%) were ELISA positive for Stx production.PCR
analysis supported the presence of one or both Shiga toxin genes (stx1
and stx2) in these isolates. Isolates M24 and M108 for stx2,  M31 and M72 were found positive for stx1,
and M91 for both genes.PCR pointing virulence factors intimin (eae) and
enterohemolysin (hlyA) showed positive amplification in all STEC
isolates (Mashkoor Mohsin, and Abdul Haque, et al., 2010).

 Diarrheal
patient samples collected from the Sindh, KPK, and Punjab Provinces, and
Islamabad that included 85 and 58, 85 and 120, 195 and  27 samples from non-flood and flood areas.Of
these samples, 39.60% (240) were positive by culture for E. coli.However,
in the months of (June–September) the surveillance period (2010–2011) from the
total of 605 samples collected in which 27.27% (165) were confirmed as E.
coli pathotypes by biochemical tests and multiplex PCR.Five E. coli
pathotypes where EPEC were 33 (28.6%), ETEC were 29 (25.2%), EAEC were 21
(18.2%) and EHEC were 5 (4.34%) based on multiplex PCR.Moreover only a single
EIEC (0.86%) and no DAEC strains were identified.EPEC was seen in Muzzafargarh,
Punjab situated close to the rivers Chenab and Indus, EAEC and ETEC were the
dominant types from Khairpur, Sindh close to the river Indus,and from Charsada,
KPK situated close to the rivers Swat and Kabul.However, Only five cases of
EHEC were detected and they were from D.I. Khan and Nowshera districts of KPK (Habib
Bokhari, and Muhammad Ali Shah et al.2013).

The study from KPK Peshawar was conducted for the
Screening of  colicinogenic gram-negative
rods against pathogenic Escherichia coli O157:H7.The data revealed that
80% of human children faeces, 100% of human adult, 90% sheep faeces
samples,100% cow/buffalo faeces samples, and 76% of septic tank water carried
GNR.From total 88 samples, 175 isolates were taken randomly and sub-cultured to
obtain pure cultures.Out of 175, 47 isolates showed antibacterial activity
against the indicator strain i.e., E. coli O157:H7. within these active
isolates, 15 human adult isolates,8 sheep isolates, 8 cow/buffalo isolates, 5
human children isolates, and 11 septic tank isolates were antagonistic towards E.
coli O157:H7 (Mushtaq, H., Bakht, J., Bacha, N.
2015).

The 92% of Sorbitol non fermenting (SNF) biotype
was detected in dung of buffaloes.Eighty percent milk and 53% beef samples
contaminated with SNF isolates of E. coli.The PCR
results shows 50% for O157, 60% for stx1 and 51%
for stx2.E. coli O157 is
a normal inhabitant of gastrointestinal tract domestic ruminant and animal
feces and acting as a primary source of contamination of beef and raw milk
(Shahzad,
K.A., et al., 2013).

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